Peptide Reconstitution Calculator — Concentration Reference
This calculator performs a laboratory concentration conversion only. It does not provide dosing information. Products supplied by Strand Peptide Research are for laboratory research use only and are not approved for human or veterinary use.
- Resulting concentration
- —
- Resulting concentration
- —
- Total mass in vial
- —
- Diluent volume for desired concentration
- —
What reconstitution means
Reconstitution is the process of dissolving a lyophilised (freeze-dried) powder in a diluent to produce a solution of known concentration for laboratory handling. The mass of material in the vial does not change; adding a measured volume of diluent simply converts that fixed mass into a liquid of defined concentration, expressed in milligrams per millilitre or micrograms per millilitre.
Choosing a diluent
Bacteriostatic water contains 0.9% benzyl alcohol as a preservative, which suppresses microbial growth in a vial that will be accessed more than once. Sterile water contains no preservative and is suited to single-use laboratory preparations. The diluent choice does not affect the concentration maths — only the volume added does. View bacteriostatic water.
Worked examples
- A 5 mg vial with 2 mL of diluent gives 5 ÷ 2 = 2.5 mg/mL (2,500 mcg/mL).
- A 10 mg vial with 2 mL of diluent gives 10 ÷ 2 = 5 mg/mL (5,000 mcg/mL).
- A 2 mg vial with 1 mL of diluent gives 2 ÷ 1 = 2 mg/mL (2,000 mcg/mL).
Accuracy and verification
The calculated concentration assumes complete dissolution of the powder and accurate measurement of the diluent volume. Undissolved material, residue left on the vial wall, or an imprecisely measured volume will all shift the true concentration away from the calculated figure. Verify the stated vial mass and purity against the batch Certificate of Analysis supplied with the product before relying on the result.
Storage after reconstitution
Once in solution, peptides are less stable than the lyophilised powder and are typically refrigerated and protected from light. Full handling and temperature guidance is set out in our peptide storage guide.